Recent advances in proteomics, biomarkers and biosensor technology sciences enable new approaches to understanding major biogeochemical processes. This project will examine the physicochemical reactivity of a model protein “RuBisCO” in seawater, and will quantify RuBisCO along ocean transect Line P (48°39.0′ N, 126°40.0′ W to 50°00′ N, 145°00′ W) in the North Pacific Ocean. The project will use two independent methods that complement and validate each other: immune-sensors and multiple-reaction monitoring (MRM) mass spectrometry.Intellectual Merit: Chemical analyses have shown that a significant fraction of dissolved organic matter (DOM) in the ocean is in the form of proteins. Proteins are a…
Posts Tagged «Monica Orellana»
Ashworth, J, S. Coesel, A. Lee, E.V. Armbrust, M.V. Orellana, and N.S. Baliga. 2013. Genome-wide diel growth state transitions in the diatom Thalassiosira pseudonana. PNAS, 110 (18) 7518–7523; doi:10.1073/pnas.1300962110.
Beer, K. D., M.V. Orellana, N.S.Baliga. 2013. Modeling the Evolution of C4 Photosynthesis. Cell: 153 (7): 1427-1429, doi:10.1016/j.cell.2013.05.058
The Arctic Data Center, supported by NSF, has highlighted Karen Junge’s work investigating rotten ice. Data has been collected on both the physical and biological properties of rotten ice and is available from the center.
This project has two main objectives: 1) determination of the physical and microbial characteristics and microstructural evolution of sea ice exposed to severe melt; and 2) exploration of the influence of biogenic particles such as sea ice algae, bacteria and polymer gels on the melting behavior of sea ice.
Frantz, C.M., Light, B., Farley, S.M., Carpenter, S., Lieblappen, R., Courville, Z., Orellana, M.V., and Junge, K.: Physical and optical characteristics of heavily melted “rotten” Arctic sea ice, The Cryosphere, 13, 775-793, doi:10.5194/tc-13-775-2019, 2019.
Kay, P., R. Choudhury, M. Nel, M.V. Orellana, P.M. Durand. 2013. Multicolour flow cytometry analyses and autofluorescence in chlorophytes: lessons from programmed cell death studies in Chlamydomonas reinhardtii. J. Appl. Phycol. 10.1007%2Fs10811-013-9988-4.
Orellana, M.V., P. Matrai, M. Janer, C, Rauchenberg. 2010. Storage of DMSP and DMS in secretory granules of Phaeocystis antarctica. J. Phycol. 47:12-117. DOI: 10.1111/j.1529-8817.2010.00936.x
Orellana, M.V., P.A. Matrai, C. Leck, C.D. Rauschenberg, A.M. Lee, E. Coz. Marine microgels as a source of cloud condensation nuclei in the high Arctic. 2011. Proc. Natl. Acad. Sci., U S A, 13612–13617, doi: 10.1073/pnas.1102457108
Orellana, M.V and D.A Hansell. 2012. RuBisCO: a long lived protein in the deep ocean. Limnol. Oceanogr., 57(3): 826–834.
Orellana, M.V., W.L. Pang, P.M. Durand, K. Whitehead, N.S. Baliga. 2013. A role for programmed cell death in the microbial loop. PLoS ONE 8(5): e62595. doi:10.1371/journal.pone.0062595.
Orellana, M.V. and C. Leck. 2014. Marine gels. In Hansel, D.A. & Carlson, C.A. Biogeochemistry of Dissolved Organic Carbon. 2nd Edition. Academic Press.
Otwell, A. E., López García de Lomana, A., Gibbons, S. M., Orellana, M. V. and Baliga, N. S. (2018), Systems biology approaches towards predictive microbial ecology. Environ Microbiol. doi:10.1111/1462-2920.14378.
The response of Arctic sea ice to a warming climate includes decreases in extent, lower ice concentration, and reduced ice thickness. Summer melt seasons are lengthening with earlier melt onsets and later autumn freezeups. We believe this will likely lead to an increase in so-called “rotten ice” in the Arctic at the end of summer. This ice has experienced a long summer of melt, is fragile, difficult to work with, and has received little attention. Comprehensive information on its physical and microbiological properties does not exist. Our team is embarking on an ambitious field campaign in order to study this poorly-understood type of sea ice in the context of its microstructural properties and potential for habitability.
Sathe, S., Orellana, M. V., Baliga, N. S. and Durand, P. M. (2019), Temporal and metabolic overlap between lipid accumulation and programmed cell death due to nitrogen starvation in the unicellular chlorophyte Chlamydomonas reinhardtii. Phycological Res., 67: 173-183. doi:10.1111/pre.12368
Skubatz, H., M.V. Orellana, W.N. Howald. 2013. A NAD(P) reductase like protein is the salicylic acid receptor in the appendix of the Sauromatum guttatum inflorescence. Intrins Disor Proteins 1:e26372; DOI: 10.4161/idp.26372
Tjernström, M., C. Lec, C. E. Birch, J. W. Bottenheim, B. J. Brooks, I. M. Brooks, L. Bäcklin, R.Y.-W. Chang, G. de Leeuw, L. Di Liberto, S. de la Rosa, E. Granath, M. Graus, A. Hanse, J. Heintzenberg, A. Held, A. Hind, P. Johnsto, J. Knulst, M. Martin, P. A. Matrai, T. Mauritsen, M. Müller, S. J. Norris, M.V. Orellana, D. A. Orsini, J. Paatero, P. O. G. Persson, Q. Gao, C. Rauschenberg, Z. Ristovsk, J. Sedlar, M. D. Shupe, B. Sierau, A. Sirevaag, S. Sjogren, O. Stetzer, E. Swietlicki, M. Szczodrak, P. Vaattovaara, N. Wahlberg, M. Westberg, and C.…
Valenzuela, Jacob J., López García de Lomana, Adrián, Lee, Allison, Armbrust, E. V., Orellana, Mónica V., Baliga, Nitin S., 2018. Ocean acidification conditions increase resilience of marine diatoms. Nature Communications, 9, 2328. https://doi.org/10.1038/s41467-018-04742-3
Mónica V. Orellana is included in The Oceanography Society’s publication of “Women in Oceanography: A Decade Later.” This supplementary issue of Oceanography includes more that 200 autobiographical sketches that provide a broad view or the types of research oceanographers conduct.