Naturally produced brominated organic compounds are ubiquitous in the oceans and are thought to be largely responsible for the formation of the Antarctic “ozone hole” in Spring. In order to accurately model and forecast global ozone and the climate, it is critical to include reactive bromine and brominated organic compounds (bromocarbons). However, bromocarbon measurements for the Antarctic are limited, especially during Spring.
Posts Tagged «bioshow_junge»
This project devises low-temperature liquid-water environments mimicking the known chemistry of brines. The research team measures microbial growth rate, metabolic activity, ability to survive while inactive, and longevity for psychrophiles to reveal proteomic biosignatures for growth, activity, and survival strategies, and understand key molecular responses of life in these environments.
Cameron, K.A., B. Hagedorn, M. Dieser, B.C. Christner, K. Choquette, R. Sletten, B. Crump, C. Kellogg, and K. Junge. 2015. Diversity and potential sources of microbiota associated with snow on western portions of the Greenland Ice Sheet. Environmental Microbiology, 17:594-609.
The Arctic Data Center, supported by NSF, has highlighted Karen Junge’s work investigating rotten ice. Data has been collected on both the physical and biological properties of rotten ice and is available from the center.
Deming, J. W. and K. Junge. ‘‘Colwellia’’, in The Proteobacteria, Part B, Bergey’s Manual of Systematic Bacteriology, G. T. Staley, D. J. Benner, N. R. Krieg, and G. M. Garrity, Eds. (Springer, New York, 2005), 2nd., Vol. 2, pp. 447–454, 2005.
Dieser, M., E.L.J.E. Broemsen, K.A. Cameron, G.M. King, A. Achberger, K. Choquette, B. Hagedorn, R. Sletten, K. Junge, and B.C. Christner. 2014. Molecular and biogeochemical evidence for methane cycling beneath the western margin of the Greenland Ice Sheet. ISME Journal, 8:2305-2316.
Ewert, M., Nunn, B. L., Firth, E., & Junge, K. (2025). Metabolic Responses, Cell Recoverability, and Protein Signatures of Three Extremophiles: Sustained Life During Long-Term Subzero Incubations. Microorganisms, 13(2), 251. https://doi.org/10.3390/microorganisms13020251
Frantz, C.M., Light, B., Farley, S.M., Carpenter, S., Lieblappen, R., Courville, Z., Orellana, M.V., and Junge, K.: Physical and optical characteristics of heavily melted “rotten” Arctic sea ice, The Cryosphere, 13, 775-793, doi:10.5194/tc-13-775-2019, 2019.
Gentilhomme, A. S., Dhakar, K., Timmins-Schiffman, E., Chaw, M., Firth, E., Junge, K., & Nunn, B. L. (2025). Proteomic Insights into Psychrophile Growth in Perchlorate-Amended Subzero Conditions: Implications for Martian Life Detection. Astrobiology. https://doi.org/10.1089/ast.2024.0065
In this pilot project (funded through the NASA Exobiology program) our Astrobiology team (PI: Karen Junge, Polar Science Center, APL, UW; postdoc: Ardith Bravenec, UW Earth and Space Science, graduate student Kaitlin Harrison, UW oceanography, both associated with the UW Astrobiology program) will join with the Navy as it conducts its biennial Ice Exercise (ICEX2024 – Operation ICE WHALE) this March (2024, see Fox News video) on sea ice off the coast of Prudhoe Bay, Alaska. We will study how microbes, temperature, and salt content affect the biological and freezing equilibrium signatures of this system with relevance to Enceladus and Europa while also training junior scientists in astrobiology-related field and laboratory work. Liquid water is essential to life as we know it.
The Saturnian moon Enceladus is a particularly promising target in the search for extraterrestrial life detection, given its large liquid ocean. While direct sample retrieval from this ocean is made difficult by the kilometers-thick ice shell surrounding it, Enceladus is host to prominent geysers that deliver the contents of this ocean to the surface (featured in this image taken during a Cassini flyby). This is a boon to life detection missions, but any life or biosignatures present in this ejecta would be exposed to the stressors of aerosolization, average surface temperatures nearing -200C, prolonged exposure to near-vacuum conditions, and UV…
Junge, K., B.C. Christner, and J.T. Staley, “Diversity of Psychrophilic Bacteria from Sea Ice – and Glacial Ice Communities“. In K. Horikoshi, G. Antranikian, A. Bull, F. Robb, and K. Stetter (eds), Extremophiles Handbook. Springer, Heidelberg, Germany. 1247 pp, 2011.
Junge K, J. J. Gosink, H.-G. Hoppe and J. T. Staley. Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic sea ice brine. Description of Planococcus mcmeekenii, sp. nov. Syst Appl Microbiol 21: 306-314, 1998.
Junge, K., C. Krembs, J. Deming, A. Stierle and H. Eicken, “A microscopic approach to investigate bacteria under in situ conditions in sea-ice samples“, Ann. Glaciol. 33: 304-310, 2001.
Junge, K., J.F. Imhoff, J.T. Staley and J.W. Deming, “Phylogenetic diversity of numerically important Arctic sea-ice bacteria cultured at subzero temperature”, Microb. Ecol. 43: 315-328, 2002.
Junge, K., H. Eicken, and J. W. Deming. “Motility of Colwellia psychrerythrea str. 34H observed at subzero temperatures“. Appl. Environ. Microbiol. 69: 4282–4284, 2003.
Junge, K., H. Eicken, and J. W. Deming. “Bacterial activity at -20°C in Arctic wintertime sea ice“. Appl. Environ. 70: 550-557, 2004.
Junge, K., H. Eicken, and J. W. Deming. A Microscopic Approach to Investigate Bacteria under In-Situ Conditions in Arctic Lake Ice: Initial Comparisons to Sea Ice. In Bioastronomy 2002: Life Amongst the Stars IAU Symposium 213, eds. R. Norris and F. Stootman. Astronomical Society of the Pacific, San Francisco: 381-388, 2004.
K. Junge, H. Eicken, B. D. Swanson and, J. W. Deming. “Bacterial incorporation of leucine into protein down to –20°C with evidence for potential activity in subeutectic saline ice formations”. Cryobiol. 52: 417–429, 2006.
Junge, K., and B.D. Swanson,’ High-resolution ice nucleation spectra of sea-ice bacteria: Implications for cloud formation and life in frozen environments‘, Biogeosciences Discussion, 4, 4261-4282, 2008.
Junge, K., Cameron, K. and Nunn, B., 2019. Diversity of Psychrophilic Bacteria in Sea and Glacier Ice Environments—Insights Through Genomics, Metagenomics, and Proteomics Approaches. In Microbial Diversity in the Genomic Era (pp. 197-216). Academic Press. https://doi.org/10.1016/B978-0-12-814849-5.00012-5
Ni Z, Arevalo R Jr, Bardyn A, Willhite L, Ray S, Southard A, Danell R, Graham J, Li X, Chou L, Briois C, Thirkell L, Makarov A, Brinckerhoff W, Eigenbrode J, Junge K, Nunn BL. (2023). Detection of Short Peptides as Putative Biosignatures of Psychrophiles via Laser Desorption Mass Spectrometry. Astrobiology, 23(6), 657-669. PubMed PMID: 37134219. https://doi.org/10.1089/ast.2022.0138.
Krembs, C., H. Eicken, K. Junge, and J. W. Deming, “High concentrations of exopolymeric substances in wintertime sea ice: Implications for the polar ocean carbon cycle and cryoprotection of diatoms“, Deep-Sea Res. I 9: 2163 –2181, 2002.
M.L. Laucks, A. Sengupta, K. Junge, E.J. Davis and B.D. Swanson. “Comparison of psychro-active Arctic marine bacteria and common mesophilic bacteria using surface-enhanced raman spectroscopy”. Appl. Spectroscopy 10: 1222-1228, 2006.
Mock, T. and K. Junge, “Psychrophilic Diatoms: Mechanisms for Survival in Freeze-thaw cycles”, Algae and Cyanobacteria in Extreme Environments, Springer Netherlands; DOI10.1007/978-1-4020-6112-7, pp. 343 to 364, 2007.
Mudge, M.C., Nunn, B.L., Firth, E., Ewert, M., Hales, K., Fondrie, W.E., Noble, W.S., Toner, J., Light, B., and Junge, K.A. (2021), Subzero, saline incubations of Colwellia psychrerythraea reveal strategies and biomarkers for sustained life in extreme icy environments. Environ Microbiol, 23: 3840-3866, doi:10.1111/1462-2920.15485.
Nunn, B.L., K. Slattery, K. A. Cameron, E. Timmins-Schiffman, and K. Junge. 2015. Proteomics of Colwellia psychrerythraea at subzero temperatures – a life with limited movement, flexible membranes and vital DNA repair. Environmental Microbiology. 111, 9009-9014.
Skidmore, M., Jungblut, Anne, and Urschel, M. and K. Junge., “Cryospheric Environments in Polar regions (Glaciers and Ice Sheets, Sea Ice, Ice Shelves).”, In Polar Microbiology. L.Whyte and R. Miller (eds). ASM Press., (2011), Accepted.
Skoog, A. K. Whitehead, F. Sperling, and K. Junge, “Microbial glucose uptake and growth along a horizontal nutrient gradient in the North Pacific“, Limnol. Oceanogr., 47(6):1676–1683, 2002.
Staley, J. T, K. Junge, and J. Deming. And some like it cold: sea ice microbiology. In Biodiversity of Life, eds. J. T Staley and A.-L. Reysenbach, pp. 423-438, 2001.