This project devises low-temperature liquid-water environments mimicking the known chemistry of brines. The research team measures microbial growth rate, metabolic activity, ability to survive while inactive, and longevity for psychrophiles to reveal proteomic biosignatures for growth, activity, and survival strategies, and understand key molecular responses of life in these environments.
Posts Tagged «bioshow_junge»
Cameron, K.A., B. Hagedorn, M. Dieser, B.C. Christner, K. Choquette, R. Sletten, B. Crump, C. Kellogg, and K. Junge. 2015. Diversity and potential sources of microbiota associated with snow on western portions of the Greenland Ice Sheet. Environmental Microbiology, 17:594-609.
The Arctic Data Center, supported by NSF, has highlighted Karen Junge’s work investigating rotten ice. Data has been collected on both the physical and biological properties of rotten ice and is available from the center.
Deming, J. W. and K. Junge. ‘‘Colwellia’’, in The Proteobacteria, Part B, Bergey’s Manual of Systematic Bacteriology, G. T. Staley, D. J. Benner, N. R. Krieg, and G. M. Garrity, Eds. (Springer, New York, 2005), 2nd., Vol. 2, pp. 447–454, 2005.
Dieser, M., E.L.J.E. Broemsen, K.A. Cameron, G.M. King, A. Achberger, K. Choquette, B. Hagedorn, R. Sletten, K. Junge, and B.C. Christner. 2014. Molecular and biogeochemical evidence for methane cycling beneath the western margin of the Greenland Ice Sheet. ISME Journal, 8:2305-2316.
Frantz, C. M., Light, B., Farley, S. M., Carpenter, S., Lieblappen, R., Courville, Z., Orellana, M. V., and Junge, K.: Physical and optical characteristics of heavily melted “rotten” Arctic sea ice, The Cryosphere, 13, 775-793, https://doi.org/10.5194/tc-13-775-2019, 2019.
This project characterizes the Greenland Ice Sheet’s subglacial microbial communities to investigate the effect of microbes on lithospheric weathering and nutrient fluxes from the GrIS margin in West Greenland.
Junge, K., B.C. Christner, and J.T. Staley, “Diversity of Psychrophilic Bacteria from Sea Ice – and Glacial Ice Communities“. In K. Horikoshi, G. Antranikian, A. Bull, F. Robb, and K. Stetter (eds), Extremophiles Handbook. Springer, Heidelberg, Germany. 1247 pp, 2011.
Junge K, J. J. Gosink, H.-G. Hoppe and J. T. Staley. Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic sea ice brine. Description of Planococcus mcmeekenii, sp. nov. Syst Appl Microbiol 21: 306-314, 1998.
Junge, K., C. Krembs, J. Deming, A. Stierle and H. Eicken, “A microscopic approach to investigate bacteria under in situ conditions in sea-ice samples“, Ann. Glaciol. 33: 304-310, 2001.
Junge, K., J.F. Imhoff, J.T. Staley and J.W. Deming, “Phylogenetic diversity of numerically important Arctic sea-ice bacteria cultured at subzero temperature”, Microb. Ecol. 43: 315-328, 2002.
Junge, K., H. Eicken, and J. W. Deming. “Motility of Colwellia psychrerythrea str. 34H observed at subzero temperatures“. Appl. Environ. Microbiol. 69: 4282–4284, 2003.
Junge, K., H. Eicken, and J. W. Deming. “Bacterial activity at -20°C in Arctic wintertime sea ice“. Appl. Environ. 70: 550-557, 2004.
Junge, K., H. Eicken, and J. W. Deming. A Microscopic Approach to Investigate Bacteria under In-Situ Conditions in Arctic Lake Ice: Initial Comparisons to Sea Ice. In Bioastronomy 2002: Life Amongst the Stars IAU Symposium 213, eds. R. Norris and F. Stootman. Astronomical Society of the Pacific, San Francisco: 381-388, 2004.
K. Junge, H. Eicken, B. D. Swanson and, J. W. Deming. “Bacterial incorporation of leucine into protein down to –20°C with evidence for potential activity in subeutectic saline ice formations”. Cryobiol. 52: 417–429, 2006.
Junge, K., and B.D. Swanson,’ High-resolution ice nucleation spectra of sea-ice bacteria: Implications for cloud formation and life in frozen environments‘, Biogeosciences Discussion, 4, 4261-4282, 2008.
Junge, K., Cameron, K. and Nunn, B., 2019. Diversity of Psychrophilic Bacteria in Sea and Glacier Ice Environments—Insights Through Genomics, Metagenomics, and Proteomics Approaches. In Microbial Diversity in the Genomic Era (pp. 197-216). Academic Press. https://doi.org/10.1016/B978-0-12-814849-5.00012-5
Krembs, C., H. Eicken, K. Junge, and J. W. Deming, “High concentrations of exopolymeric substances in wintertime sea ice: Implications for the polar ocean carbon cycle and cryoprotection of diatoms“, Deep-Sea Res. I 9: 2163 –2181, 2002.
M.L. Laucks, A. Sengupta, K. Junge, E.J. Davis and B.D. Swanson. “Comparison of psychro-active Arctic marine bacteria and common mesophilic bacteria using surface-enhanced raman spectroscopy”. Appl. Spectroscopy 10: 1222-1228, 2006.
Mock, T. and K. Junge, “Psychrophilic Diatoms: Mechanisms for Survival in Freeze-thaw cycles”, Algae and Cyanobacteria in Extreme Environments, Springer Netherlands; DOI10.1007/978-1-4020-6112-7, pp. 343 to 364, 2007.
Mudge, M.C., Nunn, B.L., Firth, E., Ewert, M., Hales, K., Fondrie, W.E., Noble, W.S., Toner, J., Light, B. and Junge, K.A., 2021. Subzero, saline incubations of Colwellia psychrerythraea reveal strategies and biomarkers for sustained life in extreme icy environments. Environmental Microbiology. https://doi.org/10.1111/1462-2920.15485
Nunn, B.L., K. Slattery, K. A. Cameron, E. Timmins-Schiffman, and K. Junge. 2015. Proteomics of Colwellia psychrerythraea at subzero temperatures – a life with limited movement, flexible membranes and vital DNA repair. Environmental Microbiology. 111, 9009-9014.
The response of Arctic sea ice to a warming climate includes decreases in extent, lower ice concentration, and reduced ice thickness. Summer melt seasons are lengthening with earlier melt onsets and later autumn freezeups. We believe this will likely lead to an increase in so-called “rotten ice” in the Arctic at the end of summer. This ice has experienced a long summer of melt, is fragile, difficult to work with, and has received little attention. Comprehensive information on its physical and microbiological properties does not exist. Our team is embarking on an ambitious field campaign in order to study this poorly-understood type of sea ice in the context of its microstructural properties and potential for habitability.
Skidmore, M., Jungblut, Anne, and Urschel, M. and K. Junge., “Cryospheric Environments in Polar regions (Glaciers and Ice Sheets, Sea Ice, Ice Shelves).”, In Polar Microbiology. L.Whyte and R. Miller (eds). ASM Press., (2011), Accepted.
Skoog, A. K. Whitehead, F. Sperling, and K. Junge, “Microbial glucose uptake and growth along a horizontal nutrient gradient in the North Pacific“, Limnol. Oceanogr., 47(6):1676–1683, 2002.
Staley, J. T, K. Junge, and J. Deming. And some like it cold: sea ice microbiology. In Biodiversity of Life, eds. J. T Staley and A.-L. Reysenbach, pp. 423-438, 2001.